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If you happen to see a question you know the answer to, please do chime in and help your fellow community members. We encourage our fourm members to be more involved, jump in and help out your fellow researchers with their questions. GATK forum is a community forum and helping each other with using GATK tools and research is the cornerstone of our success as a genomics research community.We appreciate your help!
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Mutect2 outputting samples with AD of "."
Migwell
Member ❠
I've setup a workflow that runs Mutect2, which completes successfully, but the AD of all variants is output as "."
This is an issue as we use the AD for downstream stages. Is there any reason this might be happening?
I have attached both the VCF that is missing the AD field, and the log from our workflow that includes the GATK logging. Everything there seems fine, but perhaps I'm missing something.
Tagged:
Answers
@Migwell
Hi,
Are you using the GATK4 version of Mutect2? I could not tell from the log files.
Thanks,
Sheila
No we aren't. We're using GATK 3.6:
INFO 06:31:36,894 HelpFormatter - The Genome Analysis Toolkit (GATK) v3.6-0-g89b7209, Compiled 2016/06/01 22:27:29
@Migwell
Hi,
Okay, it is best to use GATK4, as I see in my test files this issue has been fixed. You can also try a later version of GATK3, but I am not sure if this issue is fixed.
-Sheila