Attention:
The frontline support team will be unavailable to answer questions on April 15th and 17th 2019. We will be back soon after. Thank you for your patience and we apologize for any inconvenience!

Hybrid selection (exome preparation)

Hybrid selection is a method that enables selection of specific sequences from a pool of genomic DNA for targeted sequencing analyses via pull-down assays. Typical applications include the selection of exome sequences or pathogen-specific sequences in complex biological samples. Hybrid selection involve the use baits to select desired fragments.

Briefly, baits are RNA (or sometimes DNA) molecules synthesized with biotinylated nucleotides. The biotinylated nucleotides are ligands for streptavidin enabling enabling RNA:DNA hybrids to be captured in solution. The hybridization targets are sheared genomic DNA fragments, which have been "polished" with synthetic adapters to facilitate PCR cloning downstream. Hybridization of the baits with the denatured targets is followed by selective capture of the RNA:DNA "hybrids" using streptavidin-coated beads via pull-down assays or columns.

Systematic errors, ultimately leading to sequence bias and incorrect variant calls, can arise at several steps. See the GATK dictionary entry on pre-adapter artifacts and bait bias for more details.

Please see the following reference for the theory behind this technique.

Sign In or Register to comment.