What is exact meaning of filtering in vcf
Hi, I have question about output vcf using mutect2.
I run the mutect2 using only tumor set,
I got this filtering result like this,
but, the meaning of this describes in vcf,
clustered_events : clustered events (>2) observed in the tumor
panel of normals : Seen in at least 2 samples in the panel of normals
but, I confirmed the variant AF of panel of normals in EXAC database,
many variant in panel of normals don't have EXAC AF.
the description of panel of normal said "Seen" in at least 2 samples in normal,
so, Where was the variant detected in what normal database ??
after reading description of filtering, I thinks, clustered events means "tumor" and panel of normals means "normal".
but, the result in upper, the meaning of tumor and normal is in together(clustered_events;panel_of_normals ), I don't understand.
if i want to get real tumor variants, should i remove this filtering ?
but, most of variant is both meaning.
please answered this question.