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Trying to gather InsertSizeMetrics from the .bam file but getting an error.
[Mon Oct 02 18:12:59 GMT 2017] CollectInsertSizeMetrics HISTOGRAM_FILE=insert_size_histogram.pdf INPUT=input.bam OUTPUT=output_insert_size_metrics.txt DEVIATIONS=10.0 MINIMUM_PCT=0.05 METRIC_ACCUMULATION_LEVEL=[ALL_READS] INCLUDE_DUPLICATES=false ASSUME_SORTED=true STOP_AFTER=0 VERBOSITY=INFO QUIET=false VALIDATION_STRINGENCY=STRICT COMPRESSION_LEVEL=5 MAX_RECORDS_IN_RAM=500000 CREATE_INDEX=false CREATE_MD5_FILE=false GA4GH_CLIENT_SECRETS=client_secrets.json USE_JDK_DEFLATER=false USE_JDK_INFLATER=false
WARNING 2017-10-02 18:12:59 SinglePassSamProgram File reports sort order 'queryname', assuming it's coordinate sorted anyway.
WARNING 2017-10-02 18:12:59 CollectInsertSizeMetrics All data categories were discarded because they contained < 0.05 of the total aligned paired data.
WARNING 2017-10-02 18:12:59 CollectInsertSizeMetrics Total mapped pairs in all categories: 0.0
[Mon Oct 02 18:12:59 GMT 2017] picard.analysis.CollectInsertSizeMetrics done. Elapsed time: 0.00 minutes.