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VariantRecalibrator returning empty result files, no error, just "Killed"

LindsayLiangLindsayLiang Member
edited September 2017 in Ask the GATK team

Hi, I'm running the VariantRecalibrator step on a pretty small data set (50 samples in the cohort, but only for Chr21 from a whole exome sequencing project), and GATK is returning empty result files (without throwing errors), and is terminating early.

The output is as follows:

INFO  17:53:58,900 HelpFormatter - -------------------------------------------------------------------------------- 
INFO  17:53:58,908 HelpFormatter - The Genome Analysis Toolkit (GATK) v3.7-0-gcfedb67, Compiled 2016/12/12 11:21:18 
INFO  17:53:58,908 HelpFormatter - Copyright (c) 2010-2016 The Broad Institute 
INFO  17:53:58,909 HelpFormatter - For support and documentation go to https://software.broadinstitute.org/gatk 
INFO  17:53:58,909 HelpFormatter - [Thu Sep 21 17:53:58 UTC 2017] Executing on Linux 4.9.41-moby amd64 
INFO  17:53:58,910 HelpFormatter - OpenJDK 64-Bit Server VM 1.8.0_102-8u102-b14.1-1~bpo8+1-b14 
INFO  17:53:58,914 HelpFormatter - Program Args: -T VariantRecalibrator -R /vqsr_snp_model/localDir/human_g1k_v37.fasta -nt 8 -mode SNP -input /vqsr_snp_model/localDir/cohort.gt.vcf -recalFile /vqsr_snp_model/localDir/Output/cohort.gt.snp.recal.model -tranchesFile /vqsr_snp_model/localDir/Output/cohort.gt.snp.tranches -rscriptFile /vqsr_snp_model/localDir/Output/cohort.gt.snp.plots.R --use_annotation QD --use_annotation MQ --use_annotation MQRankSum --use_annotation FS --use_annotation SOR --resource:hapmap,known=false,training=true,truth=true,prior=15.0 /vqsr_snp_model/localDir/hapmap_3.3.b37.vcf --resource:omni,known=false,training=true,truth=true,prior=12.0 /vqsr_snp_model/localDir/1000G_omni2.5.b37.vcf --resource:1000G,known=false,training=true,truth=false,prior=10.0 /vqsr_snp_model/localDir/1000G_phase1.snps.high_confidence.b37.vcf --resource:dbsnp,known=true,training=false,truth=false,prior=2.0 /vqsr_snp_model/localDir/dbsnp_138.b37.vcf 
INFO  17:53:58,929 HelpFormatter - Executing as [email protected] on Linux 4.9.41-moby amd64; OpenJDK 64-Bit Server VM 1.8.0_102-8u102-b14.1-1~bpo8+1-b14. 
INFO  17:53:58,929 HelpFormatter - Date/Time: 2017/09/21 17:53:58 
INFO  17:53:58,930 HelpFormatter - -------------------------------------------------------------------------------- 
INFO  17:53:58,930 HelpFormatter - -------------------------------------------------------------------------------- 
INFO  17:53:58,983 GenomeAnalysisEngine - Strictness is SILENT 
INFO  17:53:59,163 GenomeAnalysisEngine - Downsampling Settings: Method: BY_SAMPLE, Target Coverage: 1000 
INFO  17:53:59,805 MicroScheduler - Running the GATK in parallel mode with 8 total threads, 1 CPU thread(s) for each of 8 data thread(s), of 4 processors available on this machine 
WARN  17:53:59,805 MicroScheduler - Number of requested GATK threads 8 is more than the number of available processors on this machine 4 
INFO  17:54:00,034 GenomeAnalysisEngine - Preparing for traversal 
INFO  17:54:00,042 GenomeAnalysisEngine - Done preparing for traversal 
INFO  17:54:00,043 ProgressMeter - [INITIALIZATION COMPLETE; STARTING PROCESSING] 
INFO  17:54:00,043 ProgressMeter -                 | processed |    time |    per 1M |           |   total | remaining 
INFO  17:54:00,043 ProgressMeter -        Location |     sites | elapsed |     sites | completed | runtime |   runtime 
INFO  17:54:00,054 TrainingSet - Found hapmap track:    Known = false   Training = true     Truth = true    Prior = Q15.0 
INFO  17:54:00,054 TrainingSet - Found omni track:  Known = false   Training = true     Truth = true    Prior = Q12.0 
INFO  17:54:00,055 TrainingSet - Found 1000G track:     Known = false   Training = true     Truth = false   Prior = Q10.0 
INFO  17:54:00,055 TrainingSet - Found dbsnp track:     Known = true    Training = false    Truth = false   Prior = Q2.0 
Killed

The input was:

java -Xmx12g 
-jar /usr/GenomeAnalysisTK.jar 
-T VariantRecalibrator 
-R human_g1k_v37.fasta
-nt 8 
-mode SNP
-input cohort.gt.vcf 
-recalFile cohort.gt.snp.recal.model 
-tranchesFile cohort.gt.snp.tranches 
-rscriptFile cohort.gt.snp.plots.R 
--use_annotation QD 
--use_annotation MQ 
--use_annotation MQRankSum 
--use_annotation FS 
--use_annotation SOR 
--use_annotation ReadPosRankSum
--resource:hapmap,known=false,training=true,truth=true,prior=15.0 hapmap_3.3.b37.vcf 
--resource:omni,known=false,training=true,truth=true,prior=12.0 1000G_omni2.5.b37.vcf
--resource:1000G,known=false,training=true,truth=false,prior=10.0 1000G_phase1.snps.high_confidence.b37.vcf
--resource:dbsnp,known=true,training=false,truth=false,prior=2.0" dbsnp_138.b37.vcf

Thanks!

Edit: I ran the same command again with all chromosomes of the whole exome sequences and the same error occured

Post edited by LindsayLiang on

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