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GATK 4 GenotypeGVCFs tool does not accept multiple GVCF files?

serhat_tserhat_t TurkeyMember
edited August 2017 in Ask the GATK team

I have migrated from GATK 3.7 to GATK 4 tools for my whole genome and whole exome pipelines. I am using the alignment data for a family trio (aligned by BWA-MEM). Following the best practices, I run HaplotypeCaller in GVCF mode and put all three GVCFs (for mother, father and child) through GenotpyeGVCFs tool. The error I'm getting is the following.

A USER ERROR has occurred: Argument '[V, variant]' cannot be specified more than once.

which is very strange because GenotypeGVCFs tool is designed to work with multiple GVCFs. Also, the documentation for this tool uses it exactly with multiple variant arguments.

GATK 3.7 version of GenotypeGVCFs works just fine with those arguments, so I'm currently using that. However, I would like to switch to version 4.

Is this a bug in the GATK 4 beta version?

Thanks a lot!

Best Answer


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