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# Identify haploid vs. diploid samples

University of Illinois at Urbana-ChampaignMember Posts: 2

Hello! I have RNAseq data from 40 insects with a haplodiploid sex determination system. While for pupae and adults I know the sex of the individuals, I would like to determine the sex of the younger life stages. I believe there should be a way to use SNP variants to determine whether a particular library is from a haploid (male) or diploid (female) individual, but I don't know how the GATK tools work and what would be most appropriate. I would appreciate any advice!

Tagged:

@‌beryljones

Hi,

You can call all variants assuming they are all diploid. High quality variants in haploid individuals should all be homozygous; in diploids, there will also be heterozygous calls with good quality.

Hope this helps.

-Sheila

• University of Illinois at Urbana-ChampaignMember Posts: 2
edited April 2014

Hi Sheila,

Thanks for the response! Which variant calling tool would be best? Are there any designed for transcriptome rather than genome variant discovery?

Thanks!
Beryl

• Member Posts: 31

You're asking the GATK support forum; they are going to recommend HaplotypeCaller as the strongest variant caller possible on genomic samples. The older tool, UnifiedGenotyper should also work for these purposes, but none are designed for RNA-Seq. I know when I use files aligned by TopHat there are lots of false-positive variants hanging off exon boundaries, so you should carefully select exonic locations only for this purpose.

@beryljones‌

Hi,

We do have a new updated Best Practices that explains HaplotypeCaller's abilities to handle RNA-seq data. Please refer to it here: http://www.broadinstitute.org/gatk/guide/best-practices?bpm=RNAseq

-Sheila

• MissouriMember Posts: 5

@KStamm
Could you elaborate on how you assess those false-positive variants? The frequency of SNPs we saw in our RNA-seq SNP analysis were way higher than expected and I suspect that many will be false-positives. Thank you.