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Sorry to bother you guys. Just a few quick questions:
1) I'm attempting to download the bundles for VQSR and I noticed that they are for b37 or hg19. If I performed my initial assemblies and later SNP calls with hg38, will this cause an issue? Should I restart the process using either b37 or hg19?
2) I'm still a bit lost on what is considered "too few variants" for VQSR. As VQSR works best when there are thousands of variants - is this recommendation on a per sample basis or for an entire project? I'm presently working with sequences from 80 unique samples for a single gene (~100kbp) and HaplotypeCaller detects on average ~300 raw snps. Would you recommend I hard filter instead in my case?