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Indelrealigner creates odd CIGAR

tcezardtcezard EdinburghMember Posts: 7

I'm realigning around indels with GATK v2.7.4 and in one case It's causing in one instance to align my 100 bases read long as only insertions (100I)

This might be related to another thread here but the error is slightly different.

I've uploaded the test bam files before and after realignment to your ftp

Here are the command I used to realign:

java -Xmx8G -jar GenomeAnalysisTK.jar -T RealignerTargetCreator -I test_sorted.bam -o test_sorted.intervals -R ucsc.hg19.fasta -known 1000G_phase1.indels.hg19.vcf -known Mills_and_1000G_gold_standard.indels.hg19.vcf

java -Xmx8G -jar GenomeAnalysisTK.jar -T IndelRealigner -I test_sorted.bam --out test_sorted_realigned.bam -targetIntervals test_sorted.intervals -R ucsc.hg19.fasta -known 1000G_phase1.indels.hg19.vcf -known Mills_and_1000G_gold_standard.indels.hg19.vcf




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