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Getting more runtime errors with GATK

drchriscoledrchriscole Member Posts: 16

Hi,

I had a seemingly random RUNTIME ERROR previously (mentioned on this thread http://gatkforums.broadinstitute.org/discussion/1860/runtime-error-in-baserecalibrator-version-2-2-8-g99996f2), but I'm seeing this more and more for UnifiedGenotyper runs.

It seems that every 6-10 runs I get one or two that fail as below. Re-runinng seems to work correctly, but this is annoying to find that a 12hr job has died randomly. Please can this be investigated.

ERROR ------------------------------------------------------------------------------------------
ERROR stack trace

java.lang.NullPointerException
at java.util.concurrent.locks.AbstractQueuedSynchronizer.hasQueuedPredecessors(AbstractQueuedSynchronizer.java:1453)
at java.util.concurrent.locks.ReentrantLock$FairSync.tryAcquire(ReentrantLock.java:240)
at java.util.concurrent.locks.AbstractQueuedSynchronizer.acquire(AbstractQueuedSynchronizer.java:1136)
at java.util.concurrent.locks.ReentrantLock$FairSync.lock(ReentrantLock.java:229)
at java.util.concurrent.locks.ReentrantLock.lock(ReentrantLock.java:290)
at java.util.concurrent.PriorityBlockingQueue.peek(PriorityBlockingQueue.java:286)
at org.broadinstitute.sting.utils.nanoScheduler.Reducer.reduceNextValueInQueue(Reducer.java:89)
at org.broadinstitute.sting.utils.nanoScheduler.Reducer.reduceAsMuchAsPossible(Reducer.java:120)
at org.broadinstitute.sting.utils.nanoScheduler.NanoScheduler$MapReduceJob.run(NanoScheduler.java:510)
at java.util.concurrent.Executors$RunnableAdapter.call(Executors.java:471)
at java.util.concurrent.FutureTask$Sync.innerRun(FutureTask.java:334)
at java.util.concurrent.FutureTask.run(FutureTask.java:166)
at java.util.concurrent.ThreadPoolExecutor.runWorker(ThreadPoolExecutor.java:1110)
at java.util.concurrent.ThreadPoolExecutor$Worker.run(ThreadPoolExecutor.java:603)
at java.lang.Thread.run(Thread.java:636)

ERROR ------------------------------------------------------------------------------------------
ERROR A GATK RUNTIME ERROR has occurred (version 2.2-8-g99996f2):
ERROR
ERROR Please visit the wiki to see if this is a known problem
ERROR If not, please post the error, with stack trace, to the GATK forum
ERROR Visit our website and forum for extensive documentation and answers to
ERROR commonly asked questions http://www.broadinstitute.org/gatk
ERROR
ERROR MESSAGE: Code exception (see stack trace for error itself)
ERROR ------------------------------------------------------------------------------------------

My commandline is this: java -Xmx8g -jar GenomeAnalysisTKLite.jar -nct 4 -T UnifiedGenotyper --genotype_likelihoods_model BOTH --genotyping_mode DISCOVERY -R fastafile -I infile -o outfile --dbsnp dbsnp_137.b37.vcf -stand_call_conf 30 -stand_emit_conf 30

This usually coincides with errors is calling home code, although I don't know if it's cause or effect.

"INFO 16:41:33,533 HttpMethodDirector - I/O exception (javax.net.ssl.SSLException) caught when processing request: java.lang.RuntimeException: Unexpected error: java.security.InvalidAlgorithmParameterException: the trustAnchors parameter must be non-empty

INFO 16:41:33,534 HttpMethodDirector - Retrying request
INFO 16:41:33,716 HttpMethodDirector - I/O exception (javax.net.ssl.SSLException) caught when processing request: java.lang.RuntimeException: Unexpected error: java.security.InvalidAlgorithmParameterException: the trustAnchors parameter must be non-empty
INFO 16:41:33,716 HttpMethodDirector - Retrying request
INFO 16:41:33,897 HttpMethodDirector - I/O exception (javax.net.ssl.SSLException) caught when processing request: java.lang.RuntimeException: Unexpected error: java.security.InvalidAlgorithmParameterException: the trustAnchors parameter must be non-empty
INFO 16:41:33,897 HttpMethodDirector - Retrying request
INFO 16:41:34,077 HttpMethodDirector - I/O exception (javax.net.ssl.SSLException) caught when processing request: java.lang.RuntimeException: Unexpected error: java.security.InvalidAlgorithmParameterException: the trustAnchors parameter must be non-empty
INFO 16:41:34,085 HttpMethodDirector - Retrying request
INFO 16:41:34,267 HttpMethodDirector - I/O exception (javax.net.ssl.SSLException) caught when processing request: java.lang.RuntimeException: Unexpected error: java.security.InvalidAlgorithmParameterException: the trustAnchors parameter must be non-empty
INFO 16:41:34,267 HttpMethodDirector - Retrying request "

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