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15bp deletion previously called by GATK v1 not called by GATK 2.0 or 2.1

hanalangoallenhanalangoallen Member Posts: 2
edited September 2012 in Ask the GATK team

We have a 15bp deletion in a sample that was run on an Illumina sequencer twice:

1) on GAII, 76bp paired-end reads, 10-sample pool;

2) HiSeq2000, 100bp paired-end reads, 24-sample pool.

Both times aligned with bwa and analysed with recommended GATK protocol using the latest available version. The deletion was called both times by GATK 1.0.5083 (76bp batch) and GATK 1.4 (100bp batch). However the deletion is not detected by GATK 2.0 or 2.1-8, either by UnifiedGenotyper or HaplotypeCaller. The newer GATK versions do generate fewer calls and substantially cut out false positives, but on the other hand the sensitivity seems to have dropped too much!
Here is the variant called by the GATK 1.4, 100bp batch:

7   44190538    .   CCCTCCACCCGGCCCA    C   8793.94 PASS    AC=1;AF=0.021;AN=48;BaseQRankSum=14.575;DP=7878;FS=10.160;HRun=0;HaplotypeScore=3737.9770;InbreedingCoeff=-0.0213;MQ=57.74;MQ0=0;MQRankSum=-10.741;QD=29.71;ReadPosRankSum=-0.056   GT:AD:DP:GQ:PL  0/1:216,80:296:99:8794,0,39333

And here it is called by GATK 1.0.5083, 76bp batch:

7   44190538    .   CCCTCCACCCGGCCCA    C   7765.65 PASS    AC=1;AF=0.050;AN=20;DP=1907;Dels=0.02;HRun=1;HaplotypeScore=40.0818;MQ=59.30;MQ0=0;QD=49.78;SB=-1238.06;sumGLbyD=49.78  GT:DP:GQ:PL 0/1:118:99:7766,0,14618

Where is this loss in sensitivity likely to be occurring? Can we adjust any of the default GATK settings to be able to detect these slightly larger indels?
Reads with the 15bp deletion inherently have slightly lower mapping qualities, but I would have thought this is taken into account for indel calling.
(I should add that we have a sample with a 27bp homozygous deletion and this always gets called.)

I'd be happy to send the relevant part of the bam file if that would help.

Thanks, Hana

Post edited by Geraldine_VdAuwera on


  • Geraldine_VdAuweraGeraldine_VdAuwera Administrator, Dev Posts: 10,712 admin

    Hi Hana,

    Yes, please send us the relevant part of your BAM and we'll take a look at it.

    Geraldine Van der Auwera, PhD

  • ying_sheng_1ying_sheng_1 Member Posts: 64

    I am also interesting in this issue. Any progress on this?



  • hanalangoallenhanalangoallen Member Posts: 2

    GATK v2.2 picks this deletion now so I've stopped pursuing the issue with v2.1. I haven't tested it with v2.3, but looking at the release notes most of the latest improvements are to do with performance and downsampling so I'd assume that genotyping should give pretty much the same results.


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