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Frequently Asked Questions - Specific Errors

Geraldine_VdAuweraGeraldine_VdAuwera Cambridge, MAMember, Administrator, Broadie Posts: 11,388 admin
edited September 2012 in GenomeSTRiP Documentation

1. SVGenotyper ERROR MESSAGE: Please invoke this walker with -BTI

This can be caused by an incorrect invocation of the command or a malformed input file.

The SVGenotyper walker requires the GATK -BTI argument, specifying the ROD which contains the input VCF file. Typically something like this:

-BTI input 
-B:input,VCF my_input_file.vcf

Another problem that can cause this same symptom is a malformed VCF input file. For example, if you have spaces instead of tabs in the VCF file, the GATK can fail to parse the VCF file and it can cause this error.

2. SVGenotyper ERROR MESSAGE: net.sf.picard.PicardException: Malformed query; start point 59152350 lies after end point 59128983

This can be caused by a malformed VCF file where the POS field is larger than the length of the chromosome. The "end point" value is the length of the chromosome.

Post edited by Geraldine_VdAuwera on

Geraldine Van der Auwera, PhD

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