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My current workflow for analysing mouse exome-sequencing (based on v4 of Best Practices) can require me to use slightly different VCFs as
--known parameters in BQSR, indel realignment etc. Basically, I have a "master" VCF that I subset using
SelectVariants. The choice of subset largely depends on the strain of the mice being sequenced but also on other things such as
AF'. It'd be great to be able to do this on-the-fly in conjunction with --known' in tools that required knownSites rather than having to create project-specific (or even tool-specific) VCFs.
Is there a way to do this that I've overlooked? Is this a feature that might be added to GATK?