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I'm running the Haplotype Caller on 80 bams, multithreaded (-nt 14), with either vcfs or bed files as the intervals file. I am trying to downsample to approximately 200 reads (-dcov 200). However, my output vcfs clearly have much higher depth (over 300-500 reads) in some areas. Also, HC drastically slows down whenever it hits a region of very deep pile-ups (over 1000 reads).
Is there any way to speed up HC on regions of very deep pileups? Is there a way to make downsampling stricter?
Thank you for your help.