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HI I have used indelrealinger sucessfully in the past but I am now getting the following error. Please let me know if you have any suggestions.
[Fri Feb 15 09:09:37 GMT 2013] net.sf.picard.sam.CreateSequenceDictionary REFERENCE=/exports/home/fturner/vet_roslin_ark_genomics/reference_gen omes/chicken/Gallus_gallus.WASHUC2.69.dna.toplevel.fa OUTPUT=/exports/home/fturner/vet_roslin_ark_genomics/reference_genomes/chicken/dict129865 4176074562895.tmp TRUNCATE_NAMES_AT_WHITESPACE=true NUM_SEQUENCES=2147483647 TMP_DIR=/tmp/fturner VERBOSITY=INFO QUIET=false VALIDATION_STRI NGENCY=STRICT COMPRESSION_LEVEL=5 MAX_RECORDS_IN_RAM=500000 CREATE_INDEX=false CREATE_MD5_FILE=false [Fri Feb 15 09:09:37 GMT 2013] Executing as fturner@eddie327 on Linux 2.6.32-220.23.1.el6.x86_64 amd64; Java HotSpot(TM) 64-Bit Server VM 1.7.0 _07-b10 [Fri Feb 15 09:09:52 GMT 2013] net.sf.picard.sam.CreateSequenceDictionary done. Elapsed time: 0.24 minutes. Runtime.totalMemory()=442957824
java.lang.UnsupportedOperationException: Cannot enable index memory mapping for a SAM text reader at net.sf.samtools.SAMTextReader.enableIndexMemoryMapping(SAMTextReader.java:105) at net.sf.samtools.SAMFileReader.enableIndexMemoryMapping(SAMFileReader.java:228) at org.broadinstitute.sting.gatk.datasources.reads.SAMDataSource$SAMReaders.(SAMDataSource.java:754) at org.broadinstitute.sting.gatk.datasources.reads.SAMDataSource$SAMResourcePool.createNewResource(SAMDataSource.java:729) at org.broadinstitute.sting.gatk.datasources.reads.SAMDataSource$SAMResourcePool.getAvailableReaders(SAMDataSource.java:700) at org.broadinstitute.sting.gatk.datasources.reads.SAMDataSource.(SAMDataSource.java:232) at org.broadinstitute.sting.gatk.GenomeAnalysisEngine.createReadsDataSource(GenomeAnalysisEngine.java:884) at org.broadinstitute.sting.gatk.GenomeAnalysisEngine.initializeDataSources(GenomeAnalysisEngine.java:717) at org.broadinstitute.sting.gatk.GenomeAnalysisEngine.execute(GenomeAnalysisEngine.java:207) at org.broadinstitute.sting.gatk.CommandLineExecutable.execute(CommandLineExecutable.java:104) at org.broadinstitute.sting.commandline.CommandLineProgram.start(CommandLineProgram.java:227) at org.broadinstitute.sting.gatk.CommandLineGATK.main(CommandLineGATK.java:89)
Answers
Hi there,
The version you are using is downright ancient and no longer supported... Please upgrade to the latest version.
Geraldine Van der Auwera, PhD
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