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Hi all, I am working with GATK on illumina data that was created from yeasts (SK1 strain). Since it is sequencing of a colony and not the exact same organism, I am using a filter that I wrote on the ratio of the reads that support the alternative allele. Is anyone else using something similar? Is there a build-in filter like that?
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You mean you're filtering out all reads that do not have the alternate allele?
Mauricio Carneiro, PhD http://www.broadinstitute.org/~carneiro/
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