GATK licensing moves to direct-through-Broad model -- read about it on the GATK blog


SystemSystem Posts: 226Administrator admin
edited July 2012 in Tool Bulletin

A new tool has been released!

Check out the documentation at UnifiedGenotyper.


  • vmasellivmaselli Posts: 6Member

    Hi all,
    I'm testing the GATK tools version 2.0 on two sample test and when I run UnifiedGenotyper I got a genotype only for the first sample
    I tested two different pair of samples

    Using the version 2.1, instead, I got randomly this errors:
    Unexpectedly couldn't find valid codec for temporary output file /tmp/

    Unable to parse header with error: /tmp/ (Too many open files), for input source: /tmp/

    Many thanks for any suggestion you could have


  • ebanksebanks Broad InstitutePosts: 684Member, Administrator, GATK Developer, Broadie, Moderator, DSDE Member, GP Member admin

    Please search around for answers before posting questions as you'll see that most questions have already been addressed already:

    Eric Banks, PhD -- Senior Group Leader, MPG Analysis, Broad Institute of Harvard and MIT

  • jgarbejgarbe Posts: 3Member

    The UnifiedGenotyper documentation lists "We only handle diploid genotypes" as a caveat, but non-diploid organisms have been supported since version 2.0, right?

  • Geraldine_VdAuweraGeraldine_VdAuwera Posts: 7,641Administrator, GATK Developer admin

    Yes indeed, we'll update that doc. Thanks for pointing this out.

    Geraldine Van der Auwera, PhD

  • flyingflyersflyingflyers Posts: 13Member

    Just out of curiousity, how does UnifiedGenotyper handle "Alternative hits" aligned by BWA. Sometimes BWA will provide a couple of "Alternative hits" labled as XA, attached after MD field. The "Alternative hits" have exactly the same CIGAR and Edit distance as the following read. But why the position is negative? If the mapping quality is 0, does that mean no matter where the read is placed the contribution to SNP sites are exactly the same?

    Read1 99 chr3 102778202 23 74M = 102778235 107 TTCCATCCTTTACATCCTTCTGTCTGTTCAAGAACCAGTCTGGGATCTTGCACTGGCGTGGATTCTGCATAATG __eceeceee^ec[^faadfbfgb_geeg[ba[ZdeaZ^affXbafd_dghghbaegaYZWbdbSV\\BBBB RG:Z:b_T_s_6 XT:A:R NM:i:2 SM:i:0 AM:i:0 X0:i:3 X1:i:1 XM:i:2 XO:i:0 XG:i:0 MD:Z:50T21C1 XA:Z:chr6_cox_hap1,-4623896,74M,1;chr6,-33351737,74M,1;chr6_qbl_hap2,-4429945,74M,1;

    Read2 145 chr3 32664948 0 74M chr12 23364897 0 TCTCTGCTCACTGCAACCTCCACCTCCCAGGTTCAAGCAATTGTCATGCCTCAGCCTCCTGAGTAGGTGAGATT ]]]^b^VZR`Xdc\eZ\Tdadce^aeada_fdggcc[eaZffagdddZhgfgfeadaebeZacc`[_ RG:Z:b_T_s_6 XT:A:R NM:i:4 SM:i:0 AM:i:0 X0:i:11 X1:i:0 XM:i:4 XO:i:0 XG:i:0 MD:Z:4A37C2C20C7 XA:Z:chr7,+99663378,74M,4;chr19,-49748273,74M,4;chr6,+43390671,74M,4;chr6,-166619724,74M,4;chr16,-66013198,74M,4;chr6,-33378145,74M,4;chr6_cox_hap1,-4650316,74M,4;chr6_qbl_hap2,-4456363,74M,4;chr9,-90294789,74M,4;chr4,+111287474,74M,4;

  • Geraldine_VdAuweraGeraldine_VdAuwera Posts: 7,641Administrator, GATK Developer admin

    The UG, like most if not all the GATK tools, will simply ignore alternative hits and any reads with mapping quality of zero.

    Geraldine Van der Auwera, PhD

  • xiongzhpxiongzhp Posts: 1Member

    This is my fifth time visiting this site. I still can't find what I want. I just wanna call snps. I need a simple intuitive example. All useless link and stupid docs. Can't you think like a normal person to present your tutorial?

  • Geraldine_VdAuweraGeraldine_VdAuwera Posts: 7,641Administrator, GATK Developer admin


    I understand your frustration; this is a complex topic, and we are aware we need to improve our documentation. That is an ongoing effort, and we always appreciate constructive feedback.

    However, this forum is not an appropriate channel for venting your frustration. This is a space for professionals to exchange information. Childish tantrums will not be tolerated.

    Here's a simple and intuitive piece of advice: if you would like someone to help you (for free, I might add), don't be rude. Lose the attitude and you might get what you need.

    If you don't like it, you're free to use a different software package.

    Geraldine Van der Auwera, PhD

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